The Subunit Structure and Subunit Interactions of Cytidine Triphosphate Synthetase
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Cytidine 5’-Triphosphate Synthetase of Calf Liver
Calf liver CTP synthetase was purified 2000-fold from cytosol. The formation of 1 mol of CTP from UTP was accompanied by the cleavage of 1 mol of ATP to ADP and the formation of 1 mol of L-glutamate from L-glutamine. The stoichiometry of the liver enzyme reaction was identical with that found for the Escherichia coli B enzyme (Levitzki, A., and Koshland, D. E., Jr. (1971) Biochemistry 10, 3365-...
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Cytidine triphosphate synthetase (CTP synthetase) activity was measured in extracts from normal and malignant lymphoid cells. A range of enzyme activities was found in the majority of the lymphoproliferative disorders examined, with acute lymphocytic leukemia, nodular poorly differentiated lymphocytic lymphoma, and diffuse histiocytic (large cell) lymphoma (DHL) types exhibiting the widest rang...
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Cytidine triphosphate synthetase detected earlier in crude rat liver extracts by other workers (HURLBERT, R. B., AND KAMMEN, H. O., J. Biol. Chem., 235, 443 (1960)) has been purified about 350-fold from rat and calf liver. The enzyme catalyzes the conversion of UTP to CTP in the presence of ATP, L-glutamine, Mg++ , and a sulfhydryl-containing compound. Although it is not an absolute requirement...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63424-6